Prevalence of HLA-DQ2 and HLA-DQ8 Haplotypes in adult patients of the Caja de Seguro Social in the Republic of Panama
Article Sidebar
Main Article Content
Abstract
Background: Celiac disease is a chronic, autoimmune, inflammatory disorder of the small intestine, triggered by gluten. Genetic predisposition is determined by human leukocyte antigen (HLA) haplotypes, specifically HLA-DQ2 and/or HLA-DQ8, which are present in approximately 30 % of the population; however, only about 1 % develop the disease. Its prevalence is higher in Western countries and it is frequently observed in patients with type 1 diabetes, Down syndrome, among others.
Objective: To determine the current prevalence of HLA-DQ2 and HLA-DQ8 haplotypes in adult patients.
Methods: A descriptive, observational, cross-sectional study was conducted in patients attending the Caja de Seguro Social. DNA was extracted using the DNeasy Blood kit (Qiagen) by Qiacube, and simultaneous detection was performed through end-point PCR with strip hybridization.
Results: A total of 72 patients were analyzed. Based on the results, HLA-DQ2 was the most prevalent (55 %), while HLA-DQ8 was detected in 45.3 %.
Conclusion: Our findings demonstrate that the HLA-DQ2 haplotype was the most prevalent in this study. However, the overall risk remains moderate to high for the development of celiac disease, suggesting that it may represent a potential future risk factor.
Downloads
Article Details
Issue
Section
This work is licensed under a Creative Commons Attribution-NonCommercial 4.0 International License.
How to Cite
References
1. Chanis Águila R. Características clínicas y demográficas de la enfermedad celíaca en Panamá. Pediatr Panamá. 2021;50:5-12.
2. Rojas Vargas C. Enfermedad celíaca: una enfermedad autoinmune. Rev Médica Sinerg. 2021;6.
3. Suárez Correa J, López Barreto JC, Mejía Cardona AF, Paredes Fernández AJ. Enfermedad celíaca: un reto diagnóstico en Colombia. Repert Med Cir. 2022;31:123-32.
4. Sierra M, Hernanz N, Gala I, Alonso L. Celiac disease. Med. 2020;13:9-15.
5. Ludvigsson JF, Murray JA. Epidemiology of celiac disease. Gastroenterol Clin North Am. 2019;48:1-18.
6. Aboulaghras S, Piancatelli D, Taghzouti K, Balahbib A, Alshahrani MM, Al Awadh AA, et al. Meta-analysis and systematic review of HLA DQ2/DQ8 in adults with celiac disease. Int J Mol Sci. 2023;24.
7. Operon S.A. Instrucciones kit diagnóstico enfermedad celíaca [Internet]. Zaragoza: OPERON; 2023 [citado 2023 Sep 1]. Disponible en: https://operondx.com/wp-content/uploads/pdf/instructions/09051008_CD_web.pdf
8. Martínez-Ojinaga E, et al. HLA-DQ distribution and risk assessment of celiac disease in center Spain. Rev Esp Enferm Dig. 2018;110:421-6.
9. Tye-Din JA, et al. Appropriate clinical use of human leukocyte antigen typing for coeliac disease: an Australasian perspective. Intern Med J. 2015;45:441-50.
10. Pita Fernández S, Pértega Díaz S. Asociación de variables cualitativas: test Chi cuadrado. Cadernos Aten Primaria. 2004.
11. Gudeta AN, Ramelius A, Balcha TT, Girma A, Ilonen J, Agardh D. Distribution of HLA-DQ risk genotypes for celiac disease in Ethiopian children. HLA. 2020;96:681-7. doi:10.1111/ tan.14119
12. Almeida FC, Gandolfi L, Costa KN, Picanço MRA, Almeida LM. Frequency of HLA-DQ susceptibility genotypes for celiac disease in Brazilian newborns. Mol Genet Genomic Med. 2018;6:779-84.
13. Almeida LM, Gandolfi L, Pratesi R, Harumi Uenishi R, Coutinho de Almeida F, Selleski N. Presence of DQ2.2 associated with DQ2.5 increases the risk for celiac disease. Autoimmune Dis. 2016;2016:1-6.
14. Suárez Sánchez MJ, Solano Vargas M, Zuñiga Montero M, Salazar Sánchez L. Prevalencia de los haplotipos DQ2 y DQ8 en pacientes referidos al CIHATA por estudio de enfermedad celíaca en los años 2013-2015. Costa Rica: s.n.; 2015.